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Amersham Life Sciences Inc 32p utp
32p Utp, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32p+utp/32p+utp+%CE%B1/us12583937-2044-7-8
Average 86 stars, based on 1 article reviews
32p utp - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Hybridization:

Article Title: mRNAs Encoding Telomerase Components and Regulators Are Controlled by <i>UPF</i> Genes in <i>Saccharomyces cerevisiae</i>
Article Snippet: After extraction, RNA was denatured by using glyoxal and dimethyl sulfoxide, separated on 1% agarose gels, and transferred to GeneScreen Plus (Dupont, NEN Research Products, Boston, Mass.) (4). .. RNA was detected by hybridization to radiolabeled DNA probes or riboprobes, which were prepared by in vitro transcription of template DNA in the presence of [ -32P]UTP (800 or 3,000 Ci/mmol; Amersham Life Science, Arlington Heights, Ill.) by using the Riboprobe System (Promega Corp., Madison, Wis.). ..

In Vitro:

Article Title: mRNAs Encoding Telomerase Components and Regulators Are Controlled by <i>UPF</i> Genes in <i>Saccharomyces cerevisiae</i>
Article Snippet: After extraction, RNA was denatured by using glyoxal and dimethyl sulfoxide, separated on 1% agarose gels, and transferred to GeneScreen Plus (Dupont, NEN Research Products, Boston, Mass.) (4). .. RNA was detected by hybridization to radiolabeled DNA probes or riboprobes, which were prepared by in vitro transcription of template DNA in the presence of [ -32P]UTP (800 or 3,000 Ci/mmol; Amersham Life Science, Arlington Heights, Ill.) by using the Riboprobe System (Promega Corp., Madison, Wis.). ..

Article Title: Distinct molecular mechanisms lead to diminished myelin basic protein and 2′,3′-cyclic nucleotide 3′-phosphodiesterase in qkv dysmyelination
Article Snippet: .. Antisense strand riboprobe was generated by in vitro transcription with T7 polymerase (Stratagene) in the presence of 32P-UTP (Amersham, Arlinton Heights, IL, USA) using Xho linearized BSKSCNP as DNA template. ..

Article Title: IL-18 regulates intestinal mastocytosis and Th2 cytokine production independently of IFN-gamma during Trichinella spiralis infection.
Article Snippet: A custom-made Riboquant template (BD PharMingen) was used to assay mRNA levels of IL-4, IL-13, IL-10, IL-9, IFN- , and GAPDH. .. In vitro transcription with [32P]UTP (Amersham, Little Chalfont, U.K.) was performed using a Riboprobe kit (Promega, Southampton, U.K.) and T7 polymerase (Promega). ..

Generated:

Article Title: Regulation of multiple ageing-like phenotypes by inducible klotho gene expression in klotho mutant mice.
Article Snippet: Mice carrying a loss-of-function mutation in the klotho gene (KL / mice) develop ageing-like symptoms around 4 weeks after birth and suffer from multiple age-related disorders observed in humans, including osteoporosis, arteriosclerosis, and pulmonary emphysema.. The klotho gene encodes a single-pass transmembrane protein that may function in signaling pathways that suppress ageing.. To investigate the ability of Klotho to regulate the development of ageing-related disorders, we established an inducible Klotho expression system using KL / mice carrying an exogenous klotho gene fused to the mouse metallothionein-I promoter, in which Klotho expression was dependent on zinc water feeding.

Article Title: Distinct molecular mechanisms lead to diminished myelin basic protein and 2′,3′-cyclic nucleotide 3′-phosphodiesterase in qkv dysmyelination
Article Snippet: .. Antisense strand riboprobe was generated by in vitro transcription with T7 polymerase (Stratagene) in the presence of 32P-UTP (Amersham, Arlinton Heights, IL, USA) using Xho linearized BSKSCNP as DNA template. ..

Plasmid Preparation:

Article Title: Cardiac Metallothionein Induction Plays the Major Role in the Prevention of Diabetic Cardiomyopathy by Zinc Supplementation
Article Snippet: .. The plasmid was linearized with Bgl II, and the antisense cRNA probe was produced with the use of the T7 RNA polymerase (Maxiscript kit from Ambion) in the presence of -32P UTP (Amersham). ..

Produced:

Article Title: Cardiac Metallothionein Induction Plays the Major Role in the Prevention of Diabetic Cardiomyopathy by Zinc Supplementation
Article Snippet: .. The plasmid was linearized with Bgl II, and the antisense cRNA probe was produced with the use of the T7 RNA polymerase (Maxiscript kit from Ambion) in the presence of -32P UTP (Amersham). ..

other:

Article Title: The AMPA Receptor Subunit GluR1 Regulates Dendritic Architecture of Motor Neurons
Article Snippet: Trace labeling was performed with [ 32 P]UTP (Amersham, Braunschweig, Germany) to allow calculation of yields and transcript quality check by agarose gel electrophoresis.



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